Brain imaging in zebrafish Timothy syndrome model

1. Brain morphometry Files: y680-wlt-mut-e1.zip: y680 wildtype (wlt) and homozygous mutant (mut) sibling larvae.y680-wlt-mut-e2.zip: y680 wildtype (wlt) and homozygous mutant (mut) sibling larvae. Replication experiment.y680-wlt-het.zip: y680 wildtype (wlt) and heterozygous (het) sibling larvae. Double transgenic (gad1b:RFP, tuba:mCar) 6 dpf larval zebrafish heads, scanned on confocal microscope at 1.726 x 1.726 x 2 micron resolution. Files ending -01.nii.gz are gad1b:RFP channel, -02.nii.gz are tuba:mCar channel 2. Hybridization chain reaction fluorescent in situs in 6 dpf larvae HCR-vgf-6dpf.zip: HCR for vgf in 6 dpf wild-type (wlt) and mutant (mut) larvae. Each z-stack is the mean of the indicated number of confocal images of larvae, after elastic registration to the ZBB reference brain. Resolution 1 x 1 x 1 micron. HCR-en1b-6dpf.zip: HCR for en1b in 6 dpf wild-type (wlt) and mutant (mut) larvae. Each z-stack is the mean of 9 confocal images of larvae, after affine-only registration to the ZBB reference brain. Resolution 1 x 1 x 1 micron. HCR-sox2-6dpf.zip: HCR for sox2 in 6 dpf wild-type (wlt) and mutant (mut) larvae. Each z-stack is the mean of the indicated number of confocal images of larvae, after elastic registration to the ZBB reference brain. Resolution 2 x 2 x 2 micron. 3. Expression of cacna1c during development HCR-cacna1c.zip: contains HCR for cacna1c at 2,3 and 6 dpf as indicated in the file names. Each is the mean of 5 co-registered confocal images.- HCR-cacna1c-2dpf.nii.gz: Resolution 0.86 x 0.86 x 2.0 microns.- HCR-cacna1c-3dpf.nii.gz: Resolution 0.86 x 0.86 x 2.0 microns.- HCR-cacna1c-6dpf.nii.gz: Resolution 1 x 1 x 1 microns.