Project data: Gonyostomum semen and pCO₂

This includes all data from the project "Gonyostomum semen and lake pCO2 and carbon fluxes" that has been used and referred to in the manuscript "Nuisance alga reduces lake pCO₂ and carbon flux to the atmosphere in boreal lakes". Data used in statistical analysis is found in the folder "R data" (including lake environmental data, Gonyostomum semen data and clean sensor data from which pCO₂ values were calculated). "Raw data" includes compiled data that was not used in statistical analyses, but collected during sampling and laboratory work by Karla Münzner and Thomas Rohrlack (additional lake environmental data, lake alkalinity used for pCO₂ calculations and qPCR copy number data and G. semen cell abundance calculations). The folder "Protocols qPCR" holds all lab protocols used for dna analyses (DNA extraction, DNA quantification and qPCR). There are also field notes in pdf-format for field work undertaken in Sweden ("Field notes"). The dataset was originally published in DiVA and moved to SND in 2024.本数据集包含已在论文"有害藻类降低寒带湖泊的pCO₂与大气碳通量"中使用并引用的"Gonyostomum semen与湖泊pCO₂及碳通量"项目的全部数据。用于统计分析的数据存储于"R数据"文件夹中，包含湖泊环境数据、Gonyostomum semen数据，以及用于计算pCO₂值的清洗后传感器数据。"原始数据"文件夹内存储了未用于统计分析的整合数据，这些数据由Karla Münzner与Thomas Rohrlack在采样及实验室工作中采集得到，包括额外湖泊环境数据、用于pCO₂计算的湖泊碱度数据、实时定量PCR（qPCR）拷贝数数据，以及G. semen细胞丰度计算所需数据。"qPCR实验方案"文件夹收录了所有用于DNA分析的实验室操作方案，涵盖DNA提取、DNA定量及qPCR实验流程。另有针对瑞典野外作业的PDF格式野外记录（文件名为"Field notes"）。 本数据集最初发布于DiVA平台，并于2024年迁移至SND平台。