Astrocytes Tet1 regulates neuronal development and cognition through modulating GluA1

Using RNA sequencing, we found that Tet1 alters gene expression. Mechanistically, we found that Tet1 deficiency leads to the abnormal Ca2+ signaling by regulating the expression of GluA1, which can be rescued by ectectopic expression of GluA1. Collectively, our findings suggest that Tet1 plays important functions by regulating astrocytes Ca2+ signaling. Astrocytes were isolated from two P1 Tet1 WT or KO mice and cultured in a PDL-coated T25 flask for about 7 days.