Effect of PJ-34, 5-aza-dC, TSA on HCT116 & RKO cells

In microarray analysis, combinational treatment with PJ-34 and 5-aza-dC caused dissimilar broad changes in gene expression profiles compared with their single treatments in both HCT116 and RKO cells. Profiles of reactivation of silenced genes were also different in combination of PJ-34 and 5-aza-dC and their single treatments. The results suggest that combinational use of 5-aza-dC and PARP inhibitor may be useful by causing distinct transcriptional profile changes. HCT116 & RKO cells were treated with control, single and combinational treatment with PJ-34, 5-aza-dC, TSA and microarray analysis was carried out to examine transcriptional profiles.