Screening GeCKO lentiCRISPR knockout sgRNA library in human Fireworks HeLa cells to identify components and regulators of the human nonsense-mediated mRNA decay pathway.
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资源简介：
We developed a method of in vivo amplification of NMD reporter fluorescence (Fireworks) that enables CRISPR-based forward genetic screening for NMD pathway defects in human cells. The Fireworks genetic screen identifies multiple known NMD factors and numerous human candidate genes, providing a platform for discovery of additional key factors in human mRNA degradation.
创建时间：
2016-11-13
