Individual kinetic step-size estimates (bp) for different lengths of dsDNA.

The kinetic parameters presented in this table are determined from the reaction mechanism presented in Figure 4A. Kinetic parameters are either auncorrected or bcorrected as described previously (48) by assuming that the last 10 base-pairs are unwound without direct helicase action. P is the processivity of DNA unwinding, LT is the total length of dsDNA and L0 is the minimal length of dsDNA that is stable in the presence of active helicase and with last 10 bases unpaired. The kinetic step size m is defined as the (LT−L0)/n, where n is the number of intermediates.