Single cell RNA-sequencing on healthy and IPF lung mesenchymal cells.

Single cell lung suspensions of explanted healthy and IPF donor lung tissue were generated. scRNA-seq was performed on EPCAM negative live cells sorted by FACS. Puried mesenchymal cells were clustered to evaluate the mesenchymal cell sub-clusters Overall design: To investigate pulmonary fibroblast lineage and the subtypes in human healthy and IPF lungs, mesenchymal cells (EPCAM/CD31/CD45-) were collected from five lungs from healthy donors and five lungs from IPF patients, single cell RNA-sequencing was performed on the purified mesenchymal cells.