Up-regulated PRA–target genes in the presence of P4.

MDA-iPRAB cells were cultured during 24 h in steroid-free medium containing RSL1 (0.5 μM) and/or Dox (2 μM) to induce expression of PRA and/or PRB. Cells were treated by vehicle or P4 (1 nM) for 6 hours and a genome wide transcriptomic studies was performed as described in [6]. A total of 999 genes were affected by PR expression. These genes were separated into three groups: A and B and AB (co-expressed at similar levels relative to physiological conditions) regrouping genes that are regulated by either by PRA, PRB or PRAB. Tables above represent class A+ genes regulated by PRA in the presence of the ligand; Class A+&AB+, genes responsive to liganded PRA and their expression may or may not be influenced by PRB co-expression; Class A±&AB± genes responsive to both liganded and unliganded PRA, and their expression may be influenced by PRB co-expression; Class A+&B+& AB+, genes that are commonly regulated by liganded PRA, PRB and PRAB. FC calculated either by microarray studies or by RT-qPCR analysis and their function in the cancer. FC: Transcriptional Fold Change calculated by microarray studies (FC*) and by RT-qPCR (FI, Fold Induction).
Up-regulated PRA–target genes in the presence of P4.