Polysomes

Translational profiles of wild type yeast strains were identified by microarray analysis by isolating polysomes using sucrose gradients to recover polysome-associated RNA and comparing polysome-associated RNA vs total RNA本研究通过微阵列分析（microarray analysis）鉴定了野生型酵母菌株（wild type yeast strains）的翻译谱，具体实验流程为：采用蔗糖密度梯度离心（sucrose gradients）分离多聚核糖体（polysomes）以回收多聚核糖体结合RNA（polysome-associated RNA），随后对多聚核糖体结合RNA与总RNA（total RNA）进行对比分析。