Microscopy and raw data for Alkane 1-monooxigenase gene alkB of Pseudomonas sp. FF2 is upregulated during colonisation of Arabidopsis thaliana leaves

This dataset accompanies the analysis of bacterial single-cell fluorescence and colony-forming unit (CFU) measurements in both in vitro and in planta conditions. The study focuses on the bacterial strain Pseudomonas fluorescens PFF2, evaluating its growth and promoter activity of the alkB gene using fluorescence under different growth conditions (diesel supplement growth medium and the Arabidopsis thaliana phyllosphere). The dataset supports machine learning classification models for bacterial cell identification and statistical analyses.

Codes are available at https://github.com/relab-fuberlin/alkanebioreporter

File description:

File
Data type
Description

invitro.tar.gz
Microscopy images
Images of in vitro experiments (PFF2 grown in BHB with and without diesel, and in LB)

inplanta-rep1.tar.gz
Microscopy images
Images of planta experiment 1 (PFF2 grown on Arabidopsis thaliana at 0, 2 and 7 dpi)

inplanta-rep2.tar.gz
Microscopy images
Images of planta experiment 2 (PFF2 grown on Arabidopsis thaliana at 0, 2 and 7 dpi)

in_vitro_rawdata.zip
CSV
CFU and single-cell fluorescence data of in vitro experiments

in_planta_rawdata.zip
CSV
CFU and single-cell fluorescence data of in planta experiments

trainset.zip
CSV
Ground truth-label dataset for training classification models