Diagnostic value of a nanopore sequencing assay of bronchoalveolar lavage fluid in smear-negative pulmonary tuberculosis

In this study, we analyzed the diagnostic accuracy of a nanopore sequencing assay of PCR products from a M. tuberculosis complex-specific region to detect mycobacterial pathogenic organisms in BALF and sputum samples obtained from suspected PTB patients then compared the results to MGIT culture and Xpert MTB/RIF assay results.