Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with vehicle
Assays were performed as previously described (https://doi.org/10.1016/j.cels.2022.04.005). All steps were followed by three PBS washes. Cells were fixed in 4% paraformaldehyde (EMS Emgrid 15710) for