Effect of chlorpromazine on cholesterol exit from NPC fibroblasts to serum.

In multiple replicate experiments, duplicate 75 cm2 flasks were incubated at 37°C with 10 ml DME containing 10 or 20% fetal calf serum with or without 20 µM chlorpromazine and 0.15% (i.e., ∼1 mM) HPCD. The medium was replenished after 3, 7 and 21 h of incubation as depicted in Figure 1. After 24 h, the distribution of cholesterol between the plasma membrane (PM) and intracellular (IC) pools was determined by fixing the cells and analyzing the time course of their reaction with cholesterol oxidase as described for Method B in Materials and Methods. The parenthetic values represent fractional changes; i.e., quotients of (plus CPZ)/(minus CPZ).