Comparison of Trail+/+ versus Trail-/- splenic NK cells from naive and LCMV-infected mice

Investigation of the change of the Trail-dependent NK cell transcriptome during short-term (24h) infection with lymphocytic choriomeningitis virus (LCMV). RNA sequencing-based transcriptomics analysis was performed in spleen-isolated (NK1.1+CD3-) NK cells from 3 naive Trail+/+ mice, 3 naive Trail-/- mice, 4 LCMV-infected Trail+/+ mice, and 4 LCMV-infected Trail-/- mice.本研究旨在探究淋巴细胞脉络丛脑膜炎病毒（LCMV）短期（24小时）感染过程中依赖肿瘤坏死因子相关凋亡诱导配体（Trail）的自然杀伤细胞（NK cell）转录组变化。研究从3只未致敏Trail+/+小鼠、3只未致敏Trail-/-小鼠、4只LCMV感染的Trail+/+小鼠以及4只LCMV感染的Trail-/-小鼠中分离得到脾脏来源的NK1.1+CD3- NK细胞，并对其开展基于RNA测序的转录组学分析。