VC2.2.2 blocks binding of VEGF-C to VEGF-R2 and VEGF-R3 as measured by SPR.

VEGF-R3-Fc was bound to a CM5-sensorchip coated with anti-human IgG antibody. 10 nM ΔNΔC-VEGF-C was then preincubated with a 9 to 900 times molar excess of (A) anti-VEGF-C scFv or (B) control scFv and injected on the VEGF-R3-Fc surface and the amount of binding of ΔNΔC-VEGF-C was measured by SPR. (C) VEGF-R2-Fc was bound to a CM5 sensorchip coated with anti-human IgG antibody. 10 nM of ΔNΔC-VEGF-C or VEGF-A with or without preincubation together with anti-VEGF-C scFv were then injected on the VEGF-R2 surface and bound VEGF-A or VEGF-C was measured by SPR.