scRNA-seq analysis (well-based) of pharmacological HCN channel block with ZD7288 on rat primary cortical neuronal stem cells

We analysed transcriptomic changes of single cell transcriptomes from primary rat cortical neuronal stem cells (NSC) after pharmacologically blocking HCN channels with ZD7288 compared to untreated NSC. This well-based scRNA-seq aproach revealed a pronounced cell cycle arrest in G1 phase of ZD7288 treated (HCN blocked) NSC.本研究对经ZD7288药物阻断超极化激活环核苷酸门控（HCN）通道的原代大鼠皮层神经干细胞（NSC），与未处理的NSC的单细胞转录组转录变化进行了分析。本研究采用基于微孔板的单细胞RNA测序（scRNA-seq）方法，结果显示经ZD7288处理（即HCN通道阻断）的NSC出现了显著的G1期细胞周期阻滞。