Lactobacillus crispatus Genome sequencing and assembly. Lactobacillus crispatus

Lactobacillus crispatus is a dominant organism in a healthy vaginal microbial flora. A pathological state of the vaginal flora is known as Bacterial Vaginosis (BV). The microbiome under BV is very diverse, and this state can cause several complaints and problems. The etiology of BV is unknown. Although L. crispatus is often present at low frequencies in the BV vaginal microbiome, it apparently cannot become dominant in the population, as in a healthy microbiome. A possible explanation would be that L. crispatus in BV microbiomes differs genetically from L. crispatus in healthy vaginal microbiomes. To find support for this hypothesis we will reconstruct and compare genomic sequences of L. crispatus strains isolated from healthy and BV microbiomes.Researchers at GGD Amsterdam and TNO Zeist investigate phenotypic differences of the same strains. Our task will be to provide these researchers with supporting data and leads for investigation.