Transcriptome analysis of synthetic miR-124 sponge oligo and Salmonella-treated pig PBMCs

High-density gene expression profile for synthetic miR-124 sponge oligo-treated pig PBMCs using RNA deep sequencing technology. Peripheral blood samples were collected from 20 healthy crossbreed (Duroc × Landrace × Yorkshire) piglets obtained from a commercial swine farm in Jingzhou, China either treated with Salmonella Typhi along or with miR-124 sponge + Salmonella Typhi without sacrificing the animals (ten males and ten females, four weeks old). At 12 h post treatment, cells were collected and total RNA was isolated. Sequencing libraries were prepared with an Illumina Truseq RNA sample preparation kit according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Single-read sequencing (read length: 50 bp) was conducted on an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA).