Eumelanin and pheomelanin content in wild-type and PMEL−/− mice on mice on different genetic backgrounds.

The animals depicted in Figure 6 were analyzed.a4-Amino-3-hydroxyphenylalanine (4-AHP), a degradation product of pheomelanin. Pheomelanin content was estimated by multiplying 4-AHP by a factor of 9 [36]. Based on a single determination per animal.bPyrrole-2,3,5-tricarboxylic acid (PTCA), a degradation product of eumelanin [33]. Eumelanin content was estimated by multiplying PTCA content by a factor of 25. Based on duplicate assays from a single animal/genotype.