Energetic metabolites, digestive enzymes, detoxification and oxidative stress indicators of Octopus mimus females and embryos

Morphological changes of Octopus mimus females were evaluated during ovarian development. Digestive gland (DG) and ovaries wet weight were assessed to obtain gonadosomatic and hepatosomatic indices. Obtained from a group of females placed in tanks until spawn, embryos were incubated and sampled during development. Females (DG and ovaries), embryos (at different stages of development) and paralarvae (one and three days old) were preserved for energetic metabolites (glucose, glycogen, cholesterol, triacylglycerides, and protein concentrations), digestive enzyme activities (acidic proteases, alkaline proteases, trypsin and lipases), detoxification (acetylcholinesterase and carboxylesterase activities), and oxidative stress indicators (catalase activity, glutathione-s-transferase activity, superoxide dismutase activity, redox potential, total glutathione and lipid peroxidation).