PCBP-1 regulates the transcription and alternative splicing of inflammation and ubiquitination-related genes in Parkinson's disease model

In this study, we cloned and constitutively overexpressed PCBP-1 in rat PC12 cells. RNA-seq was performed to analyze PCBP-1-regulated differentially expressed genes (DEGs) and alternative splicing events (ASEs) between control and PCBP1-overexpressed cells. GO and KEGG pathway analyses were performed to identify functional DEGs and alternatively spliced genes. Consequently, we validated PCBP-1-regulated genes using RT-qPCR.Overexpression of PCBP-1 widely regulated the ASE and expression of genes enriched in neuroinflammation and protein ubiquitination, which were also associated with PD pathogenesis. Moreover, RT-qPCR assay verified the PCBP-1-modulated expression of neuroinflammatory genes, like LCN-2, and alternative splicing (AS) of ubiquitination-related gene WWP-2 Overall design: Detecting the expression level of differential genes and alternative splicing after PCBP1 overexpression in PC12 cells by RNA-seq