miRNA-seq analysis using Arabidopsis Col-0 WT and hasty KO mutant

To examine the effect of hasty KO mutation on miRNA expression, we performed miRNA-seq analysis using Arabidopsis Col-0 WT and hasty KO mutant that were grown under control and low nitrogen conditions. Shoot tissues from 10 day-old Col-0 WT and hasty KO mutants that were initially grown under standard nutrient conditions for 5 days and then grown under low nitrogen conditions for 5 days were used for miRNA-seq analysis.