Toll signalling controls intestinal regeneration in Drosophila

This study is part of a manuscript approved for publication in Development (Cambridge UK) showing that the Toll pathway in Drosophila is necessary and sufficient for intestinal stem cell mitosis following infection and under homeostatic conditions. Chronic activation of Toll using a gain of function mutation (Toll10b) induces intestinal stem cell proliferation while blocking differentiation of enteroblasts to enterocytes. Thus, the intestinal tissue becomes replete with progenitor cells. The RNA-seq data deposited here are on whole guts of 20-day old flies where Toll has been activated in intestinal progenitor cells post-developmentally (in adults) from day 1 using the GAL4/GAL80ts system. Overall design: Whole guts were dissected from flies with the following genotypes: esgts/w1118 (control genetic background), esgts/UAS-Toll10b (overactivation of the Toll pathway), esgts/UAS-Toll10b/UAS-BskDN (blocked overactivity of the Toll pathway by a dominant negative form of JNK). Libraries were generated using the MGIEasy RNA-seq Library Preparation Universal Kit in BGI HongKong. RNA-seq was performed on the DNBSEQ-G400 using the 150 paired-end sequencing protocol. 3 samples (esgts/w1118, esgts/UAS-Toll10b, esgts/UAS-Toll10b/UAS-BskDN) with 3 independent biological repeats were used.