Transcription profiling by array of human MCF7 breast cancer cells with FOXP3 target gene expression with and without MOF knockdown

As we clarified before, FOXP3 gene is an X-linked tumor suppressor for both human and mouse. And we also clarified direct target genes of FOXP3 in human cancer cells. Recently we identified that MOF, a histone acetyltransferase, can be a FOXP3's molecular partner to regulate target gene expression. We conducted a microarray analysis using MCF7 cell, a human breast cancer cell line, with FOXP3-tet-off system with and without MOF knock down before and after FOXP3 overexpression. After 48 hrs of RNAi treatments in MCF7 cells, FOXP3 was overexpressed and those cells were cultured for additional 48 hrs. Total RNA were extracted using Qiagen's RNeasy column and were applied to Affymetrix Human U133 Plus 2.0 array according to the manufacture's protocol.