Total proteins identified and quantified in the <i>T</i>. <i>cruzi-</i>infected SH-SY5Y cells with and without <i>ST8sia2</i> gene silencing.
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SH-SY5Y cells were seeded in 6-well microplates (1 x 106 cells/well), and previously treated with siRNA ST8Sia2 [100 nM] for 24h before infection, followed by infection with T. cruzi trypomastigotes (Y strain) at ratio of 1:5 (SH-SY5Y:trypomastigotes). After 24 h.p.i, T. cruzi-infected SH-SY5Y cells were treated with siRNA ST8Sia2 [100 nM] for an additional 24h. Then, the cells were lysed in 8M urea containing protease inhibitors using a sonicator for three cycles at 10% amplitude, reduced, alkylated and digested. The tryptic peptides were analysed by nanoflow liquid chromatography coupled to mass spectrometry to map the differentially proteomic profile in response to T. cruzi infection. A triplicate for each condition was run. The total proteins identified in the two conditions along with their quantitative values in each replicate. (XLSX)
