Vicia faba cultivar:Hassawii 2 Transcriptome or Gene expression. Vicia faba cultivar:Hassawii 2

Drought and salinity are the major factors that limit faba bean production worldwide. Elucidating stress related genes will allow trait and crop manipulation and facilitate the production of stress tolerant elite genotypes. The main objective of the current project was to performed whole transcriptome analyses of drought tolerant Hasaawi 2 genotype, to better understand the molecular basis of stress response/adaptation. RNA-seq NGS technology was used to analyze samples of roots and leaves both at vegetative and reproductive stages under both control and drought induced conditions. In this project a total 167.25 Gb bases was generated after Illumina Hiseq sequencing. After assembly, unigenes were subjected for functional annotation with 7 functional databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro). The highest annotated unigenes were obtained for Nt- and Nr databases which were 55.07 % and 54.82 % respectively while lowest were obtained with GO- data base. These annotated unigenes were classified into different GO catagories with 36.31% (38852) unigenes were for Biological processes, 29.42 % (31477) unigenes for cellular components and 34.26% (36661) unigenes for molecular function. The unigenes were also subjected to SSRs identification. In total, 15291 potential SSR motifs were identified and the majority belonged to trinucleotide (7620) and dinucleotide (6073) repeats. The unigeness thus obtained were further processed for gene expression analysis for identification of drought responsive genes. A pairwise comparison revealed divergent gene expression in the Hassawi 2 genotype at different developmental stages in toots and leaves. A total of 651 unigenes were up-regulated and only 11 were down regulated at flowering stage of leaves. However, at vegetative stage, a large number of unigenes were down regulated, 27803 unigenes were up-regulated and 17430 were down-regulated. In roots, majority of unigenes were down regulated at both developmental stages. At flowering stage, 19071 unigenes were up regulated while 24631 genes were down regulated in roots at flowering stage. However 89 unigenes were up regulated in roots and 1787 were down regulated. The results of this report will be further integrated with detailed bioinformatics analysis and also incorporated by quantitative PCR analysis of drought resistance genes in final progress report. These genes could be used for trait manipulation for development of faba bean genotypes with enhanced drought tolerance and adoption to the local environments.