mRNA recovery from total RNA.

Polyadenylated mRNA isolated from total RNA from three biological replicates of cells grown under continuous white light (CWL), 48 h dark-treated cells (D48) and cells re-exposed to white light (WL) for 0.5, 6 and 24 h. Equal amounts of total RNA was used as starting material. aThe mRNA concentration for one of the biological replicates for the dark-treated cultures were <0.20 ng/µl (<8 ng in total), which is out of range for the Qubit fluorometer. The value for this replicate was therefore set to 8 ng in the further calculations.