scRNA-seq and spatial transcriptomics of MIBC across different ICB exposure and response state

To explore how TME components regulate tumor cell fate plasticity through intricate cellular interaction networks, and thereby induce ICB resistance in MIBC, we performed scRNA-seq on 11 tumor samples obtained from 6 MIBC patients, covering distinct anatomical regions. Additionally, we conducted spatial transcriptomics on freshly collected specimens from 4 of these patients. We performed multiple bioinformatic analysis and employed in vitro functional assays to validate our findings.