Next Generation Sequencing of human first trimester placental EVTs (pEVT), decidual interstitial EVTs (iEVT), and trophoblast organoid (TB-ORG)-derived extravillous trophoblasts (TB-ORG-EVTs).

Aim of the study was to verify signaling pathway-provoked EVT specification in human first trimester placental and decidual tissue. We further provide improvement for in-vitro differentiated TB-ORG-EVTs to more closely mimic in-situ placental EVT geno- and phenotypes. Overall design: In the first batch, in-situ placental, and decidual, and in-vitro-derived TB-ORG-EVTs from first trimester tissue (7 - 8th weeks of gestation) were isolated and generated, respectively, from the same single donors. In the second batch TB-ORG-EVTs were differentiated under three conditions: a) DIFF1: conventional, transforming growth factor beta (TGFb)-inhibiting condition, b) DIFF2: without TGFb inhibition; c) DIFF3: without TGFb inhibition and recombinant TGFb1