Plasmids and vectors.
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*) Chromosomal positions from S. aureus N315 are used. The RNase J1 gene is in a putative operon with an upstream transcriptional regulator (SA0941), and both genes were included in the pJ1 plasmid, in order to include the promoter and to avoid the problems with RNase J1 expression described by Mäder and coworkers [8].
创建时间:
2015-12-02



