Global quadriceps skeletal muscle transcript data from fasted male BXD strains on chow or high fat diet

Transcript data from quadriceps skeletal muscle from fasted-state male BXD strains on Quadriceps, Chow or Quadriceps, High fat diet We used microarrays to compare the skeletal muscle expression differences across males in the BXD strain family and across two diverse diets 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused, then quadriceps were snap-frozen in liquid nitrogen for RNA extraction and RNEasy cleanup. Each dietary and strain cohort consisted of ~5 animals which were prepared independently then pooled evenly by µg RNA before the Affymetrix arrays were run.本数据集源自喂食普通饲料（Chow）或高脂饲料（High fat diet）的禁食状态雄性BXD品系（BXD strain）小鼠的股四头肌骨骼肌转录组数据。本研究采用基因芯片（microarray）技术，对比BXD品系家族雄性小鼠间以及两种不同饮食条件下的骨骼肌基因表达差异。实验对象为29周龄雄性小鼠，于当日下午6时至次日上午9时禁食过夜，经异氟烷（isoflurane）麻醉后实施灌流，随后摘取股四头肌组织并置于液氮中快速冷冻，用于后续RNA提取及RNEasy纯化。每个饮食-品系队列包含约5只独立制备的小鼠，在开展Affymetrix基因芯片检测前，将每只小鼠的RNA按微克数等量混合后再进行芯片实验。