DataSheet7_Matrine, a potential c-Myc inhibitor, suppresses ribosome biogenesis and nucleotide metabolism in myeloid leukemia.xlsx

Previous studies have shown that matrine, a natural compound extracted from the herb Sophora flavescens, has a good anti-leukemia effect, but its key target and mechanism remains unclear. Here, we found that only c-Myc could respond rapidly to matrine treatment in three myeloid leukemia cell lines, and matrine inhibited both transcription and translation of c-Myc. Ribosome biogenesis and nucleotide metabolism, the key downstream of c-Myc, were significantly suppressed after matrine treatment. Therefore, our results confirmed that matrine is a special c-Myc inhibitor which suppresses ribosome biogenesis and nucleotide metabolism by inhibiting c-Myc in myeloid leukemia. This study provides scientific basis for the development of matrine derivatives to c-Myc-driven cancers.既往研究表明，从 Sophora flavescens 草本植物中提取的自然化合物——土大黄素，具有良好的抗白血病作用，但其关键靶点及作用机制尚不明确。本研究发现，在三种髓系白血病细胞系中，仅 c-Myc 可迅速响应土大黄素治疗，且土大黄素可抑制 c-Myc 的转录与翻译。经过土大黄素处理后，c-Myc 的重要下游效应——核糖体生物合成和核苷酸代谢显著受到抑制。因此，我们的研究证实土大黄素是一种特殊的 c-Myc 抑制剂，通过抑制髓系白血病中的 c-Myc 来抑制核糖体生物合成和核苷酸代谢。本研究为开发针对 c-Myc 驱动的癌症的土大黄素衍生物提供了科学依据。