Localization of attachment organelle components determined by immunogold EM.

The cells expressing N-terminus EYFP-fusion proteins were treated by 0.3% Triton X-100, chemically fixed, and sequentially labeled by antiserum against EYFP and the secondary antibody conjugated with a 5-nm gold particle. Bold and slim core structures are shown in the left and the right panels, respectively, for each protein. Histograms show the distributions of the gold positions measured along the axis and these positions are traced by Gaussian distribution shown by a black line. The bar of the histogram is colored according to the categories shown in Fig 3. The peak positions from the terminus and the number of examined cores are shown in each panel.