A linear relationship was tested between blue dye accumulations and feeding frequency using ANOVA in linear mixed effects model.

The P value of the interaction terms is also displayed, which indicated whether the regression coefficients differ between comparative conditions (NF = no. of flies per condition and NV = no. of vials per condition). aThese assays were not repeated on different trial dates. The statistical analysis was therefore only on fixed effects, i.e., a regression analysis. bThese standard errors are for the differences in the intercepts.