Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction. Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

we assessed the impact of TLR3 L412F at endogenous cellular levels upon double stranded RNA (dsRNA) stimulation, by performing RNA-sequencing in primary fibroblasts from individuals homozygous for the ancestral allele (n =2) or homozygous for TLR3 L412F (n = 4) upon extracellular Poly(I:C) stimulation that activates the TLR3 pathway. The average response of TLR3 L412F homozygotes was significantly decreased (mean Δ log2 fold-change = 0.93; Wilcoxon p <10-16) compared to that of cells with the ancestral allele, approaching that of TLR3-deficient cells, used as negative control. Overall design: Comparative gene expression profiling analysis of RNA-seq data for primary human fibroblasts of individuals with the TLR3 412F derived allele and its ancestral complementary allele at the same genomic position