Linear standard curves for the PC gene and Cm insertion.
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ay = Ct value (PCR cycle value at the fluorescence threshold of 0.1 for PC gene and 0.08 for Cm insertion), x = amount of template DNA (expressed as cell number equivalents),bAmplification efficiencies (E) were calculated as follows E = (10−1/slope−1)×100.
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2015-12-02



