Isolation and Characterization Bifidobacterium from Kishu-Narezushi.

Bifidobacterum was isolated from Kishu-Narezushi, a traditional Japanese naturally fermented food consisting of fish, rice, and salt. The isolated Bifidobacterium was identified as Bifidobacterium psychraerophilum and named the Yasuke strain.The Yasuke strain and Bifidobacterium psychraerophilum DSM22366 were more resistatnt to salt and oxygen than that of Bifidobacterium strains.The salt torelance of the Yasuke strain was characterized in B.psychraeriphilum.Draft genome analysis of the Yasuke strain and the DSM22366 strain suggest that B. psychraerophilum has a characteristic genes related to stress tolerance,although no distinctive gene was found in Yasuke strain.The B.psychraerophilum Yasuke strain is the first Bifidobacterium isolated from a traditional Japanese naturally fermented food.