Characterization of CS/D product.
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A, Purity of CS/D final product analyzed by silver-stained SDS-PAGE. Lanes, 1, 2, 3 and 4 show 2 µg, 1 µg, 0.5 µg, 0.25 µg of CS/D, respectively; M, molecular weight marker. B, Western blot using host cell protein polyclonal antibodies. Lane 1 shows 2 µg CS/D, lane 2 shows 1 µg CS/D; Ec, whole cell E. coli lysate positive control; M, molecular weight marker. C, Size-exclusion HPLC elution profile of CS/D. D, Mass spectrometry analysis of CS/D. E, Thermal Stability of CS/D shown on a non-reduced SDS-PAGE with temperature and incubation time shown above the gel. F, Stability of CS/D following a freeze-thaw cycle. Frozen and thawed samples (Thaw) were centrifuged (Spun) were analyzed by coomassie-stained, reduced (R) and non-reduced (N) SDS-PAGE and by mouse polyclonal anti-CS/D western blot.
创建时间：
2014-10-24
