Development of gene expression signatures using herbal drug ninjin'yoeito (NYT)

To identify hematopoietic genes regulated by NYT, microarray analysis was carried out to compare BM MNCs cultured with and without NYT for 11 days. BM MNCs cultured with NYT or negative controls were collected at day 11. Total RNA was extracted using RNeasy Plus Micro Kit (QIAGEN). RNA concentration was measured by NANODROP 2000c (Thermo Fisher Scientific). cRNA was amplified, labeled, and hybridized to an Agilent Whole Mouse GE 4 9 44K v2 Microarray (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions. All hybridized microarrays were scanned using an Agilent scanner, and signals of all probes were analyzed using Feature Extraction Software (Agilent Technologies).