Analysis of RA vs 4-oxo-RA gene expression regulation in Xenopus laevis

We analyzed anterior halves of NF stage 15 embryos that were treated with 10uM DMSO (control), or 1uM RA and 10uM 4-oxo-RA (experimental) for changes in gene expression induced by the two mophogens. RNA-sequencing revealed significant overlap in genes upregulated by both RA and 4-oxo-RA, and to a lesser extent, those downregulated by them. We report all Zic-1 targets described in accompanying manuscript to be regulated by both RA and 4-oxo-RA. Examination of gene expression changes induced by RA and 4-oxo-RA in anterior regions of neurulating NF stage 15 Xenopus laevis embryos