Transcription profiling of mouse liver cells during the circadian cycle at 4 hour time resolution

Cyclic regulatory systems are ubiquitous in cells and tissues. In the liver rhythms in mRNA expression are determined by the homeostatic regulation that operates on daily circumstances. In particular the specific response to nutrients, as well as systemic and peripheral circadian oscillators, contribute to the set up of the hepatic homeostasis at different phases of the day. In this series we used microarrays to detail the global program of gene expression in the mouse liver under physiological daily variations, determined by both the feeding and the circadian cycles. We examined 7 samples, each consisting in a pool of RNAs extracted from the whole liver of 5 mice. Samples were collected at times ZT2, ZT6, ZT10 ,ZT14, ZT18, ZT22, ZT26 (ZT0/24 and ZT12 are the times when the lights are switched on and off, respectively)循环调控系统广泛存在于细胞与组织中。肝脏内的mRNA表达节律由适配日常生理状态的稳态调控所决定。 具体而言，机体对营养物质的特异性应答，以及中枢与外周生物钟振荡器，共同促成了一日内不同时段肝脏稳态的建立。 本系列实验采用基因芯片（microarrays）详细解析了由进食周期与生物钟周期共同调控的生理性每日节律变化下，小鼠肝脏内的全局基因表达调控程序。 本次实验共设置7份样本，每份样本均为5只小鼠全肝提取的RNA混合池。 样本分别于ZT2、ZT6、ZT10、ZT14、ZT18、ZT22、ZT26时刻采集（其中ZT0/24与ZT12分别对应灯光开启与关闭的时刻）。