GNPS - Parallel host and microbial pathways regulate lipid desaturation
收藏NIAID Data Ecosystem2026-05-01 收录
下载链接：
https://www.omicsdi.org/dataset/gnps/MSV000092700
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资源简介：
4 datasets, each in their own subfolder.
1) acdh-11 comparative metabolomics. 4 genotypes (N2, fcmt-1, WT FAT-7::GFP, and acdh-11;FAT-7::GFP) grown at 3 temperatures (15, 20, 25). Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately.
2) acdh-11 comparative metabolomics re: diet. 2 genotypes (WT FAT-7::GFP, and acdh-11;FAT-7::GFP) and 2 diets (WT E. coli, BW25113, and cyclopropane-deficient E. Coli, JW1653-1). The cyclopropane-deficient strain was additionally supplemented with cyclopropane fatty acids lactobacillic acid (LBA) or dihydrosterculic acid (DHSA) at 20 uM.
3) fcmt-1 comparative metabolomics. 4 genotypes (N2, fcmt-1(gk155709), fcmt-1(tm2382), hacl-1(tm6725)) grown at 20C. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately.
4) vaccenic acid isotope labeling. 2 genotypes (N2 and hacl-1(tm6725)) grown at 20C. Each genotype was supplemented with vehicle, cis-vaccenic acid (cis-VA), stable isotope labeled D13-cis-VA, trans-vaccenic acid (trans-VA), and stable isotope labeled D13-trans-VA. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately.
创建时间：
2023-08-20
