Transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan

Serotonin is a monoamine that regulates processes such as energy balance and immune function. Manipulating this pathway in growing dairy calves could promote growth and development by modulating functions and signaling pathways within key organs. In this study, we characterized the adipose and muscle transcriptome of pre-weaned calves with increased serotonin bioavailability through the elucidation of differentially expressed genes. Holstein bull calves (21d old) were fed milk replacer (8 L/d) supplemented with saline (CON, n=4) or 5-hydroxytryptophan (5HTP, n=4) for 10 d. Calves were euthanized on d 10 to harvest adipose and muscle tissue for RNA-Sequencing.