DataSheet1_Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks.docx

Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive, time-resolved monitoring of the culture status in shake flasks are lacking. The Respiration Activity Monitoring Respiration Activity Monitoring System system was used to determine the oxygen transfer rate (OTR) in shake flasks. It was proven that the OTR could be regarded as equal to the oxygen uptake rate as the change of the dissolved oxygen concentration in the liquid phase over time was negligibly small. Thus, monitoring the oxygen transfer rate (OTR) was used to increase the information content from shake flask experiments. The OTR of a Chinese hamster ovary cell line was monitored by applying electrochemical sensors. Glass flasks stoppered with cotton plugs and polycarbonate flasks stoppered with vent-caps were compared in terms of mass transfer characteristics and culture behavior. Similar mass transfer resistances were determined for both sterile closures. The OTR was found to be well reproducible within one experiment (standard deviation

哺乳动物细胞的培养通常在摇瓶中进行。与仪器化生物反应器相比，摇瓶中非侵入性、时间分辨的监测培养状态的选择方案尚显不足。本研究采用呼吸活性监测系统，以测定摇瓶中的氧气传递率（OTR）。经证实，OTR可视为与氧摄取率相等，因为随时间推移液体相中溶解氧浓度的变化可忽略不计。因此，通过监测氧气传递率（OTR）可提高摇瓶实验的信息含量。通过应用电化学传感器，对中国汉仓鼠卵巢细胞系的OTR进行了监测。比较了带有棉塞的玻璃瓶和带有通风盖的聚碳酸酯瓶在传质特性和培养行为方面的差异。对于两种无菌封闭，确定了相似的传质阻力。发现OTR在单一实验中具有良好的重现性（标准偏差 ...)
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