Gene expression profile at single cell level of mononuclear cells from the liver sinusoid. Gene expression profile at single cell level of mononuclear cells from the liver sinusoid

This study identified a distinct liver sinusoidal NK-like CD56hiCD161-CD8+ T-cell population that expands in the liver with hepatitis B virus-associated chronic liver disease. CD56hiCD161-CD8+ T cells exert NKG2C-mediated NK-like effector functions even without TCR stimulation. Overall design: We obtained liver sinusoidal mononuclear cells from the liver perfusate of healthy donors and recipients with hepatitis B virus (HBV)-associated chronic liver disease (CLD) during liver transplantation. We performed a CITE-seq analysis of liver sinusoidal CD45+ cells in combination with TCR-seq of liver sinusoidal mononuclear cells. Please note that several samples were pooled into 1 GEM, and hashtags were used to distinguish each sample; lsmc1_1: hashtag1 (HBV 1) lsmc1_2: hashtag4 (HBV 2) lsmc2_1: hashtag1 & hashtag4 (HC1 & 2) lsmc2_2: hashtag2 & hashtag3 (HC3 & 4) lsmc2_3: hashtag1 & hashtag4 (HBV 3 & 4) lsmc2_4: hashtag2 (HBV 5)