Glomerulonephritis in BXSB/MpJ-Yaa mice

Analysis of glomeruli isolated from kidneys of 4 month male BXSB/MpJ-Yaa. The BXSB/MpJ-Yaa mouse strain is a lupus-prone model. Results provide insight into the pathogenic mechanisms linked to glomerulonephritis in BXSB/MpJ-Yaa mice. The glomeruli were isolated from 6 BXSB/MpJ-Yaa (glomerulonephritis model) and BXSB/MpJ-Yaa+ (control). 6 glomerular populations of each strain were devided into 3 groups (1 group / 2 populations). 3 glomerulonephritis models versus 3 controls analysis was performed. BXSB/MpJ-Yaa is a recombinant inbred strain developed originally from a C57BL/6 female mouse and a SB/Le male mouse. Yaa (Y-linked autoimmune accelerator locus) localizing on the Y chromosome of BXSB/MpJ-Yaa male mice is the result of a duplication of an about 4 Mbp telomeric segment near the pseudoautosomal region of the X chromosome onto the Y chromosome. BXSB/MpJ-Yaa+ (control) male mice carry the wild-type Y chromosome in place of the mutant Yaa-containing Y chromosome of BXSB/MpJ-Yaa male mice.本研究对4月龄雄性BXSB/MpJ-Yaa小鼠肾脏分离的肾小球开展分析。BXSB/MpJ-Yaa小鼠品系是狼疮易感模型，本研究结果可为解析该品系小鼠肾小球肾炎的致病机制提供参考。实验所用肾小球分别分离自6只BXSB/MpJ-Yaa（肾小球肾炎模型组）小鼠与6只BXSB/MpJ-Yaa+（对照组）小鼠：每个品系的6个肾小球群体被分为3组（每组包含2个群体），最终完成了3个模型组与3个对照组的对比分析。BXSB/MpJ-Yaa是一种重组近交系小鼠，最初由C57BL/6雌性小鼠与SB/Le雄性小鼠培育获得。Yaa（Y连锁自身免疫加速位点，Y-linked autoimmune accelerator locus）定位于BXSB/MpJ-Yaa雄性小鼠的Y染色体上，其形成机制为X染色体拟常染色体区域附近约4 Mbp的端粒片段发生重复，并易位至Y染色体。BXSB/MpJ-Yaa+（对照组）雄性小鼠携带野生型Y染色体，而非BXSB/MpJ-Yaa雄性小鼠所携带的、带有突变Yaa的Y染色体。