RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

The ability ofMycobacterium tuberculosis(Mtb) to tolerate nitric oxide (NO) and superoxide (O2•-) produced by phagocytes contributes to its success as a human pathogen. Recombination of NO and O2•- generates peroxynitrite (ONOO­-­), a potent oxidant produced inside immunologically-activated macrophages and causes lethality in diverse organisms. However, while the response ofMtbtowards NO and O2•- is well established, howMtbresponds to ONOO­- remains uncertain. Filling this knowledge gap is important to understand the persistence mechanisms of Mtb during infection Hence, we used an endogenous peroxynitrite donor CJ067 and treated Mtb to identify new mechanisms of ONOO­-­ resistance in mycobacteria. Overall design: This study involves determining the mRNA transcriptome profiles for the strains Mtb H37Rv untreated and treated with 2X MIC CJ067; grown under standard in vitro conditions in 7H9+ADS+Tween80 at 37°C and 180 rpm, till cultures reached ~0.4 OD600. Three independent experiments were done for each treatment.