Quantification of glial activity at the lumbar level of spinal cord in bone cancer after 14 days.

Fluorescence immunohistochemistry was performed and quantified in dorsal spinal cord (L1-L3) of cancer-bearing and sham rats (n = 8). IBa1 immunolabeling of microglia showed a significant increase in fluorescence intensity between ipsilateral and contralateral sides (#; p<0.05) and between carcinoma-implanted animals and sham-group ipsilateral sides (*; p<0.05). GFAP immunostaining of astrocytes did not reveal any significant difference in fluorescence intensity thresholds between groups.