RNA-Seq of sorted E10.5 and E11.5 AGM cell populations from Gata3-GFP embryos

The aim of this study was to examine the whole transcriptome of pools of 20 cells of Gata3-expressing AGM subpopulations (haematopoietic [HC], mesenchymal [MC], sympathetic nervous system [SNS], and endothelial [EC]) and compare them to their non-Gata3 expressing counterparts Cells were sorted into: Gata3+ EC: GFP+ Cdh5+ CD41- CD45-, Gata3- EC: GFP- Cdh5+ CD41- CD45-, Gata3+ HC: GFP+ CD41+ CD45+, Gata3- HC: GFP- CD41+ CD45+, Gata3+ MC: GFP+ Pdgfrb+, Gata3- MC: GFP- Pdgfrb+, Gata3+ SNS: GFP+ Ngfr+ and Gata3- SNS: GFP- Ngfr+. Each population was then sorted into the lysis buffer in a 96-well plate at 20 cells per well.