Unveiling the intracellular stress responses, introduced and induced by higher level of glutathione using S. cerevisiae

Glutathione (GSH) is a thiol and low-molecular-weight tripeptide consisted of Glu, Cys, and Gly. GSH is the most predominant anti-oxidant among various thiol molecules, and widely exists with milli molar level in prokaryotes and eukaryotes including mammalian. Importantly, the levels of GSH and ratio GSH/GSSG are steadily regulated to proceed or maintain many essential intracellular activities, i.e., GSH homeostasis. Many researches so far have proven that GSH-depleted cells can be responsible with aging, tumor, type-II diabetes, or neurodegenerative. While, as little research has been done that higher level of GSH could be loaded acutely onto cells (GSH stress), there is no clue how cells respond to and what factor would engage with GSH stress. In this study, we intend to partially unveil GSH stress-responsive mechanism using S. cerevisiae for model of mammalian cell. Here we performed RNA-seq analysis, and tried to screen pivotal factors dependent on GSH-stress response in yeast.